Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking.

@article{Iijima2005ComprehensiveSA,
  title={Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking.},
  author={Manabu Iijima and Tomonori Hashimoto and Yasuyuki Matsuda and Taku Nagai and Yuichiro Yamano and Tomohiko Ichi and Tsukasa Osaki and Shun-ichiro Kawabata},
  journal={The FEBS journal},
  year={2005},
  volume={272 18},
  pages={4774-86}
}
Arthropod cuticles play an important role as the first barrier against invading pathogens. We extensively determined the sequences of horseshoe crab cuticular proteins. Proteins extracted from a part of the ventral side of the cuticle were purified by chitin-affinity chromatography, and separated by two-dimensional SDS/PAGE. Proteins appearing on the gel… CONTINUE READING