Comprehensive screening procedure for diuretics in urine by high-performance liquid chromatography.

@article{Cooper1989ComprehensiveSP,
  title={Comprehensive screening procedure for diuretics in urine by high-performance liquid chromatography.},
  author={Syretha F. Cooper and Ren{\'e} Masse and Robert Dugal},
  journal={Journal of chromatography},
  year={1989},
  volume={489 1},
  pages={65-88}
}
A rapid and reliable screening procedure using high-performance liquid chromatography for the detection of 23 diuretics (belonging to five different pharmacological groups) in urine has been developed. Two aliquots of 2-ml urine samples were extracted separately under acidic and basic conditions. The acidic and basic extracts were pooled, evaporated to dryness and reconstituted in methanol. The methanolic extract was injected onto a Hewlett-Packard Hypersil ODS C18 (5 microns) column (column I… CONTINUE READING

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