Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing.

@article{Breci2005ComprehensivePI,
  title={Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing.},
  author={Linda A. Breci and Emily Hattrup and Matthew L Keeler and Jessica Letarte and Roxanne L Johnson and Paul A Haynes},
  journal={Proteomics},
  year={2005},
  volume={5 8},
  pages={2018-28}
}
We have investigated the use of a variety of different techniques to identify as many proteins as possible in a yeast lysate, with the aim of investigating the overlap and complementarity of data from different approaches. A standard lysate was prepared from log phase yeast (Saccharomyces cerevisiae). This was then subjected to analysis via five different… CONTINUE READING