Comprehensive microRNA expression profiling of the hematopoietic hierarchy.

  title={Comprehensive microRNA expression profiling of the hematopoietic hierarchy.},
  author={Oleh I. Petriv and Florian Kuchenbauer and Allen D. Delaney and V{\'e}ronique Lecault and Alanna White and David Kent and Luis F. Marmolejo and Michael Heuser and Tracey Berg and Megan Copley and Jens Ruschmann and Sanja Sekulovic and Claudia Benz and Etsushi Kuroda and Victor Ho and Frann Antignano and T Halim and Vincenzo Giambra and Gerald Krystal and Chiharu Takei and Andrew P. Weng and James M. Piret and Connie G Eaves and Marco A. Marra and Richard Keith Humphries and Carl L. Hansen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={107 35},
The hematopoietic system produces a large number of highly specialized cell types that are derived through a hierarchical differentiation process from a common stem cell population. miRNAs are critical players in orchestrating this differentiation. Here, we report the development and application of a high-throughput microfluidic real-time quantitative PCR (RT-qPCR) approach for generating global miRNA profiles for 27 phenotypically distinct cell populations isolated from normal adult mouse… CONTINUE READING