Comprehensive comparative analysis of strand-specific RNA sequencing methods

  title={Comprehensive comparative analysis of strand-specific RNA sequencing methods},
  author={Joshua Z. Levin and Moran Yassour and Xian Adiconis and Chad Nusbaum and Dawn A Thompson and Nir Friedman and Andreas Gnirke and Aviv Regev},
  booktitle={Nature Methods},
Strand-specific, massively parallel cDNA sequencing (RNA-seq) is a powerful tool for transcript discovery, genome annotation and expression profiling. There are multiple published methods for strand-specific RNA-seq, but no consensus exists as to how to choose between them. Here we developed a comprehensive computational pipeline to compare library quality metrics from any RNA-seq method. Using the well-annotated Saccharomyces cerevisiae transcriptome as a benchmark, we compared seven library… CONTINUE READING
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