Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

@inproceedings{Hemmerich2014ComprehensiveCS,
  title={Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase},
  author={Johannes Hemmerich and N{\'u}ria Adelantado and Jos{\'e} Manuel Barrig{\'o}n and Xavier Ponte and Astrid H{\"o}rmann and Pau Ferrer and Frank Kensy and Francisco Valero},
  booktitle={Microbial cell factories},
  year={2014}
}
BackgroundIn Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (PAOX1), induced by methanol, present low reproducibility leading to high time and resource consumption.ResultsAn automated microfermentation platform (RoboLector) was successfully tested to overcome the chronic problems of clone selection and optimization of fed-batch strategies. Different clones from Mut+P… CONTINUE READING
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