Comprehensive chronic lymphocytic leukemia diagnostics by combined multiplex ligation dependent probe amplification (MLPA) and interphase fluorescence in situ hybridization (iFISH)

@article{Alhourani2014ComprehensiveCL,
  title={Comprehensive chronic lymphocytic leukemia diagnostics by combined multiplex ligation dependent probe amplification (MLPA) and interphase fluorescence in situ hybridization (iFISH)},
  author={Eyad Alhourani and Martina Rin{\vc}i{\'c} and Moneeb A. K. Othman and Beate Pohle and Cordula Schlie and Anita Glaser and Thomas Liehr},
  journal={Molecular Cytogenetics},
  year={2014},
  volume={7}
}
BackgroundBanding-karyotyping and metaphase-directed-fluorescence-in-situhybridization (FISH) may be hampered by low mitotic index in leukemia. Interphase FISH (iFISH) is a way out here, however, testing many probes at the same time is protracted and expensive. Here multiplex-ligation-dependent-probe-amplification (MLPA) was used retrospectively in chronic lymphocytic leukemia (CLL) samples initially studied by banding cytogenetics and iFISH. Detection rates of iFISH and MLPA were compared and… 

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