Comprehensive and reliable phosphorylation site mapping of individual phosphoproteins by combination of multiple stage mass spectrometric analysis with a target-decoy database search.

@article{Han2009ComprehensiveAR,
  title={Comprehensive and reliable phosphorylation site mapping of individual phosphoproteins by combination of multiple stage mass spectrometric analysis with a target-decoy database search.},
  author={Guanghui Han and Mingliang Ye and Xinning Jiang and Rui Chen and Jian Ren and Yu Xue and Fangjun Wang and Chunxia Song and Xuebiao Yao and Hanfa Zou},
  journal={Analytical chemistry},
  year={2009},
  volume={81 14},
  pages={
          5794-805
        }
}
Since the emergence of proteomics, much attention has been paid to the development of new technologies for phosphoproteomcis analysis. Compared with large scale phosphorylation analysis at the proteome level, comprehensive and reliable phosphorylation site mapping of individual phosphoprotein is equally important. Here, we present a modified target-decoy database search strategy for confident phosphorylation site analysis of individual phosphoproteins without manual interpretation of spectra… 

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