Comprehensive and cost-effective NMR spectroscopy of methyl groups in large proteins.

@article{Otten2010ComprehensiveAC,
  title={Comprehensive and cost-effective NMR spectroscopy of methyl groups in large proteins.},
  author={Renee Otten and Byron C. H. Chu and Karla D. Krewulak and Hans J. Vogel and Frans A. A. Mulder},
  journal={Journal of the American Chemical Society},
  year={2010},
  volume={132 9},
  pages={2952-60}
}
An NMR approach is described which yields the methyl resonance assignments of alanine, threonine, valine, leucine, and isoleucine residues in proteins with high sensitivity and excellent resolution. The method relies on protein samples produced by bacterial expression using [(1)H,(13)C]-D-glucose and approximately 100% D(2)O, which is cost-effective and ensures the isotopic enrichment of all possible methyl groups. Magnetization transfer throughout the methyl-containing side chains is possible… CONTINUE READING

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