Comprehensive analysis of mitochondria in roots and hypocotyls of soybean under flooding stress using proteomics and metabolomics techniques.

@article{Komatsu2011ComprehensiveAO,
  title={Comprehensive analysis of mitochondria in roots and hypocotyls of soybean under flooding stress using proteomics and metabolomics techniques.},
  author={Setsuko Komatsu and Aiichiro Yamamoto and Takuji Nakamura and Mohammad-Zaman Nouri and Yohei Nanjo and Keito Nishizawa and Kiyoshi Furukawa},
  journal={Journal of proteome research},
  year={2011},
  volume={10 9},
  pages={
          3993-4004
        }
}
Flooding is a serious problem for soybeans because it reduces growth and grain yield. Proteomic and metabolomic techniques were used to examine whether mitochondrial function is altered in soybeans by flooding stress. Mitochondrial fractions were purified from the roots and hypocotyls of 4-day-old soybean seedlings that had been flooded for 2 days. Mitochondrial matrix and membrane proteins were separated by two-dimensional polyacrylamide gel electrophoresis and blue-native polyacrylamide gel… CONTINUE READING
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