Composition of the essential oil from cell suspension cultures of Achillea millefolium ssp. millefolium

Abstract

The composition of the essential oil isolated from Achillea millefolium L. ssp. millefolium cell suspension cultures was analysed by GC and GC-MS. The yield of the oil obtained by hydrodistillation or a simultaneous distillation -extraction of these cultures, harvested at days 8–10 (end of exponential phase), was 0.001 % (w/w). The analysis of the volatiles showed the presence of thirteen components; monoterpenes amounted to 5%, sesquiterpene hydrocarbons attained 40%, while eugenol, demethoxyencecalin and two unidentified compounds amounted to 45% of the total oil. Several methods were tested in an attempt to increase the essential oil production by the cultures: growth on solid medium, growth in light, use of a different culture medium, elicitation with cellulase or yeast extract, and growth in a two-phase system. Of the different methods tested, the growth in B5+ medium with Miglyol 812 led to the highest essential oil yield (0.002%, w/w), and resulted in a more diverse oil composition.

DOI: 10.1007/BF00037663

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@article{Figueiredo2004CompositionOT, title={Composition of the essential oil from cell suspension cultures of Achillea millefolium ssp. millefolium}, author={Ana Cristina da Silva Figueiredo and Marc Salom{\'e} and Salom{\'e} Pais and Johannes J. C. Scheffer}, journal={Plant Cell, Tissue and Organ Culture}, year={2004}, volume={40}, pages={113-118} }