Composition of Perineuronal Net Extracellular Matrix in Rat Brain

@article{Deepa2006CompositionOP,
  title={Composition of Perineuronal Net Extracellular Matrix in Rat Brain},
  author={Sarama Sathyaseelan Deepa and Daniela Carulli and Clare M. Galtrey and Kate E. Rhodes and Junko Fukuda and Tadahisa Mikami and Kazuyuki Sugahara and James W. Fawcett},
  journal={Journal of Biological Chemistry},
  year={2006},
  volume={281},
  pages={17789 - 17800}
}
We developed a method to extract differentially chondroitin sulfate proteoglycans (CSPGs) that are diffusely present in the central nervous system (CNS) matrix and CSPGs that are present in the condensed matrix of perineuronal nets (PNNs). Adult rat brain was sequentially extracted with Tris-buffered saline (TBS), TBS-containing detergent, 1 m NaCl, and 6 m urea. Extracting tissue sections with these buffers showed that the diffuse and membrane-bound CSPGs were extracted in the first three… 

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