Composition of Perineuronal Net Extracellular Matrix in Rat Brain

  title={Composition of Perineuronal Net Extracellular Matrix in Rat Brain},
  author={Sarama Sathyaseelan Deepa and Daniela Carulli and Clare M. Galtrey and Kate E. Rhodes and Junko Fukuda and Tadahisa Mikami and Kazuyuki Sugahara and James W. Fawcett},
  journal={Journal of Biological Chemistry},
  pages={17789 - 17800}
We developed a method to extract differentially chondroitin sulfate proteoglycans (CSPGs) that are diffusely present in the central nervous system (CNS) matrix and CSPGs that are present in the condensed matrix of perineuronal nets (PNNs). Adult rat brain was sequentially extracted with Tris-buffered saline (TBS), TBS-containing detergent, 1 m NaCl, and 6 m urea. Extracting tissue sections with these buffers showed that the diffuse and membrane-bound CSPGs were extracted in the first three… 

Tables from this paper

Experience-dependent development of perineuronal nets and chondroitin sulfate proteoglycan receptors in mouse visual cortex.
  • Qian YeQing-long Miao
  • Biology
    Matrix biology : journal of the International Society for Matrix Biology
  • 2013
Perineuronal net, CSPG receptor and their regulation of neural plasticity.
Perineuronal nets (PNNs) are reticular structures resulting from the aggregation of extracellular matrix (ECM) molecules around the cell body and proximal neurite of specific population of neurons in
Upregulation of aggrecan, link protein 1, and hyaluronan synthases during formation of perineuronal nets in the rat cerebellum
Analysis of immunohistochemistry and in situ hybridization data suggest that aggrecan, HA, and, particularly, Crtl1 might be crucial elements for the initial assembly of PNNs.
Brain ageing changes proteoglycan sulfation, rendering perineuronal nets more inhibitory
It is suggested that the change in PNN GAG sulfation in aged brains renders the PNNs more inhibitory, which lead to a decrease in plasticity and adversely affect memory.
Proteoglycan Sulphation in the Function of the Mature Central Nervous System
  • J. FawcettJ. Kwok
  • Biology, Computer Science
    Frontiers in Integrative Neuroscience
  • 2022
These functions of CSPGs and HSPGs are strongly influenced by the pattern of sulphation of the glycan chains, the sulphation code, which governs the binding properties and biological effects of the proteoglycans.
Extracellular matrix and perineuronal nets in CNS repair
A perineuronal net (PNN) is a layer of lattice‐like matrix which enwraps the surface of the soma and dendrites, and in some cases the axon initial segments, in sub‐populations of neurons in the


Composition of perineuronal nets in the adult rat cerebellum and the cellular origin of their components
It is proposed that HASs, which can retain HA on the cell surface, may act as a link between PNNs and neurons, and HAS and link proteins might be key molecules for PNN formation and stability.
Molecular interactions of neural chondroitin sulfate proteoglycans in the brain development.
This review mainly summarizes recent studies on the involvement of these three classes of proteoglycan in cell-cell and cell-substratum interactions during the brain development.
Degradation of Chondroitin Sulfate Proteoglycans Induces Sprouting of Intact Purkinje Axons in the Cerebellum of the Adult Rat
The results show that, in the absence of injury or novel external stimuli, degradation of chondroitin sulfate proteoglycans is sufficient to induce Purkinje axon sprouting but not the formation of long-lasting synaptic contacts.
On the existence of a cartilage‐like proteoglycan and link proteins in the central nervous system
The perineuronal matrix of the central nervous system consists, in part, of a hyaluronate‐bound aggrecan‐like proteoglycan and link proteins, and that the former is produced by astrocytes.
Functional characterization of chondroitin sulfate proteoglycans of brain: interactions with neurons and neural cell adhesion molecules
The results indicate that certain brain proteoglycans can bind to neurons, and that Ng-CAM and N-Cam may be heterophilic ligands for neurocan and the 3F8 proteoglycan.
Proteoglycans in the developing brain: new conceptual insights for old proteins.
This review summarizes the most recent data on structures and functions of brain proteoglycans and focuses on new physiological concepts for their potential roles in the developing central nervous system.
Extracellular matrix components associated with remodeling processes in brain
  • U. Rauch
  • Biology
    Cellular and Molecular Life Sciences CMLS
  • 2004
Components of the extracellular matrix could provide valuable early information about the pathological state of the brain and their implication in different types of remodeling processes in adult brain, like normal and pathological synaptic plasticity, inflammatory and dementia-associated diseases and gliomas are indicated.