Components of the yeast spindle and spindle pole body

@article{Rout1990ComponentsOT,
  title={Components of the yeast spindle and spindle pole body},
  author={Michael P. Rout and John V. Kilmartin},
  journal={The Journal of Cell Biology},
  year={1990},
  volume={111},
  pages={1913 - 1927}
}
Yeast spindle pole bodies (SPBs) with attached nuclear microtubles were enriched approximately 600-fold from yeast cell extracts. 14 mAbs prepared against this enriched SPB fraction define at least three components of the SPB and spindle. Immunofluorescent staining of yeast cells showed that throughout the cell cycle two of the components (110 and 90 kD) were localized exclusively to the SPB region, and the other (80 kD) was localized both to the SPB region and to particulate dots in short… CONTINUE READING
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