Complexome profiling identifies TMEM126B as a component of the mitochondrial complex I assembly complex.

@article{Heide2012ComplexomePI,
  title={Complexome profiling identifies TMEM126B as a component of the mitochondrial complex I assembly complex.},
  author={Heinrich Heide and Lea Bleier and Mirco Steger and J{\"o}rg Ackermann and Stefan Dr{\"o}se and Bettina Schwamb and Martin Z{\"o}rnig and Andreas S. Reichert and Ina Koch and Ilka Wittig and Ulrich Brandt},
  journal={Cell metabolism},
  year={2012},
  volume={16 4},
  pages={538-49}
}
Macromolecular complexes are essential players in numerous biological processes. They are often large, dynamic, and rather labile; approaches to study them are scarce. Covering masses up to ∼30 MDa, we separated the native complexome of rat heart mitochondria by blue-native and large-pore blue-native gel electrophoresis to analyze its constituents by mass spectrometry. Similarities in migration patterns allowed hierarchical clustering into interaction profiles representing a comprehensive… CONTINUE READING