Complexity in evolved regulatory variation for alcohol dehydrogenase genes in HawaiianDrosophila

  title={Complexity in evolved regulatory variation for alcohol dehydrogenase genes in HawaiianDrosophila},
  author={X M Fang and Chyong‐Yi Wu and Mark D. Brennan},
  journal={Journal of Molecular Evolution},
SummaryThe alcohol dehydrogenase gene (Adh gene) ofDrosophila affinidisjuncta is expressed at a higher level in the larval midgut and Malpighian tubules than the homologous gene fromDrosophila hawaiiensis. This study analyzed thecis-acting sequences responsible for these regulatory differences in larval tissues ofDrosophila melanogaster transformants. A series of 10 chimeric and deletedAdh genes was introduced into the germ line ofD. melanogaster, and tissue-specific expression levels were… 
Similar tissue-specific expression of the Adh genes from different Drosophila species is mediated by distinct arrangements of cis-acting sequences
Comparison of these results with earlier studies involving chimeras between the D. affinidisjuncta and D. hawaiiensis genes indicates that expression in the adult midgut is influenced by multiple regulatory sequences and that distinct arrangements of regulatory sequences can result in similar levels of expression both in theadult midGut and in the whole organism.
Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships
Adh and Adh-dup evolve differently as shown by the synonymous and nonsynonymous substitution rates for the coding region of both genes when compared across two species of the melanogaster group, two of the obscura group of the subgenus Sophophora and D. lebanonensis of the victoria group ofTheSubgenus Scaptodrsophila.
Evolution of cis-regulatory sequence and function in Diptera
Biochemical characterization and in vivo assays of cis-regulatory sequences on a genomic-scale will relieve the barrier for computational identification and interpretation of cis-, structure-function relationship of these sequences.


Tissue-specific regulatory differences for the alcohol dehydrogenase genes of Hawaiian Drosophila are conserved in Drosophila melanogaster transformants.
Analysis of larval transformants of Hawaiian picture-winged Drosophila indicates that demonstrable differences in cis-dominant regulatory information are sufficient to account for the observed regulatory variation.
Nucleotide sequence of the genomic region encoding alcohol dehydrogenase inDrosophila affinidisjuncta
DNA sequence and RNA sequences from Drosophila affinidisjuncta and Adh transcripts support the conclusion that two Adh promoters generate distinct, developmentally regulated transcripts, and putative homologies occur in the two promoter regions.
Isolation and initial characterization of the alcohol dehydrogenase gene from Drosophila affinidisjuncta.
Recombinant bacteriophages containing the alcohol dehydrogenase (ADH) gene from Drosophila affinidisjuncta have been isolated by virtue of their cross-hybridization to the previously cloned ADH gene
Two alternative transcripts coding for alcohol dehydrogenase accumulate with different developmental specificities in different species of picture-winged Drosophila.
Two alternate transcripts of the single copy Alcohol dehydrogenase (Adh) gene accumulate with developmental specificity in all of 12 species of Hawaiian picture-winged Drosophila which have been examined, document the existence of a novel type of genetic variability and suggest specific properties for the biological systems that regulate ADH expression in Drosphila.
Identification of cis-regulatory elements required for larval expression of the Drosophila melanogaster alcohol dehydrogenase gene.
It is shown that transcription from the proximal promoter of the melanogaster Adh gene is regulated by a far upstream enhancer and two or more elements near the transcription start site, and each of the identified regulatory elements is sufficient for low levels of AdH gene expression in all three larval tissues, but maximal levels of expression requires the entire set.
Complex cis-acting regulatory genes demonstrated in Drosophila hybrids
The distribution of activity among all the three forms of these dimeric enzymes produced in hybrids indicates that the pattern differences reflect differential accumulation of enzyme molecules, not altered catalytic properties.
Evolution of patterns of gene expression in Hawaiian picture-wingedDrosophila
The results provide strong direct evidence for rapid evolution of new patterns of gene regulation in this group of organisms and the adaptive significance of these pattern differences is unknown.
Developmentally regulated RNA transcripts coding for alcohol dehydrogenase in Drosophila affinidisjuncta.
The organization of the gene coding for alcohol dehydrogenase (Adh) in Drosophila affinidisjuncta has been determined by physically mapping Adh RNA transcripts to cloned genomic DNA. Two distinct
Tissue-specific expression phenotypes of Hawaiian Drosophila Adh genes in Drosophila melanogaster transformants.
Interspecific differences in the tissue-specific patterns of expression displayed by the alcohol dehydrogenase (Adh) genes within the Hawaiian picture-winged Drosophila represent a rich source of