Complexity in evolved regulatory variation for alcohol dehydrogenase genes in HawaiianDrosophila

@article{Fang2006ComplexityIE,
  title={Complexity in evolved regulatory variation for alcohol dehydrogenase genes in HawaiianDrosophila},
  author={X M Fang and Chyong‐Yi Wu and Mark D. Brennan},
  journal={Journal of Molecular Evolution},
  year={2006},
  volume={32},
  pages={220-226}
}
SummaryThe alcohol dehydrogenase gene (Adh gene) ofDrosophila affinidisjuncta is expressed at a higher level in the larval midgut and Malpighian tubules than the homologous gene fromDrosophila hawaiiensis. This study analyzed thecis-acting sequences responsible for these regulatory differences in larval tissues ofDrosophila melanogaster transformants. A series of 10 chimeric and deletedAdh genes was introduced into the germ line ofD. melanogaster, and tissue-specific expression levels were… 
Similar tissue-specific expression of the Adh genes from different Drosophila species is mediated by distinct arrangements of cis-acting sequences
TLDR
Comparison of these results with earlier studies involving chimeras between the D. affinidisjuncta and D. hawaiiensis genes indicates that expression in the adult midgut is influenced by multiple regulatory sequences and that distinct arrangements of regulatory sequences can result in similar levels of expression both in theadult midGut and in the whole organism.
Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships
TLDR
Adh and Adh-dup evolve differently as shown by the synonymous and nonsynonymous substitution rates for the coding region of both genes when compared across two species of the melanogaster group, two of the obscura group of the subgenus Sophophora and D. lebanonensis of the victoria group ofTheSubgenus Scaptodrsophila.
Evolution of cis-regulatory sequence and function in Diptera
TLDR
Biochemical characterization and in vivo assays of cis-regulatory sequences on a genomic-scale will relieve the barrier for computational identification and interpretation of cis-, structure-function relationship of these sequences.

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TLDR
Analysis of larval transformants of Hawaiian picture-winged Drosophila indicates that demonstrable differences in cis-dominant regulatory information are sufficient to account for the observed regulatory variation.
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TLDR
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TLDR
Two alternate transcripts of the single copy Alcohol dehydrogenase (Adh) gene accumulate with developmental specificity in all of 12 species of Hawaiian picture-winged Drosophila which have been examined, document the existence of a novel type of genetic variability and suggest specific properties for the biological systems that regulate ADH expression in Drosphila.
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TLDR
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TLDR
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TLDR
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