Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.

@article{Monteiro1997ComplexPA,
  title={Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.},
  author={Lurdes Monteiro and D Bonnemaison and Antoine Vekris and Klaus G. Petry and Julien Bonnet and Roberto Vidal and Jos{\'e} Cabrita and Francis M{\'e}graud},
  journal={Journal of clinical microbiology},
  year={1997},
  volume={35 4},
  pages={995-8}
}
A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet. 

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