Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry.

@article{Ciminiello2010ComplexPP,
  title={Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry.},
  author={P. Ciminiello and C. Dell’Aversano and Emma Dello Iacovo and E. Fattorusso and M. Forino and L. Grauso and L. Tartaglione and F. Guerrini and R. Pistocchi},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2010},
  volume={24 18},
  pages={
          2735-44
        }
}
Over the past decades, Italian coastlines have been plagued by recurring presence of the benthic dinoflagellate Ostreopsis ovata. Such an alga has caused severe sanitary emergencies and economic losses due to its production of palytoxin-like compounds. Previous studies have confirmed the presence of ovatoxin-a (OVTX-a) as the major toxin of the algal toxin profile together with small amounts of putative palytoxin (PLTX). In our ongoing research on O. ovata toxins we report herein on in-depth… Expand
Unique toxin profile of a Mediterranean Ostreopsis cf. ovata strain: HR LC-MS(n) characterization of ovatoxin-f, a new palytoxin congener.
TLDR
The presence of ovatoxin-f should be taken into account when monitoring programs for palytoxin-like compounds in microalgae and/or seawater are carried out. Expand
The novel ovatoxin-g and isobaric palytoxin (so far referred to as putative palytoxin) from Ostreopsis cf. ovata (NW Mediterranean Sea): structural insights by LC-high resolution MSn
TLDR
Tentative structures were assigned to both ovatoxin-g and the putative palytoxin, the latter being identified as a palyToxin isomer and re-named as  isobaric paly toxin. Expand
Isolation and structure elucidation of ovatoxin-a, the major toxin produced by Ostreopsis ovata.
TLDR
The present paper reports on the isolation, NMR-based structural determination, and preliminary mouse lethality evaluation of ovatoxin-a, the major toxic compound contained in O. cf. Expand
First Identification of Palytoxin-Like Molecules in the Atlantic Coral Species Palythoa canariensis.
TLDR
It is demonstrated that Palythoa canariensis produces a mixture of PLTX-like molecules, of special relevance to safeguard human health considering PalythOA species are commonly used for decoration by aquarium hobbyists. Expand
Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification
TLDR
Toxin extracts obtained from collected biomass were purified using different techniques such as liquid-liquid partition or size exclusion, and open-column chromatography with Sephadex LH20 phase yielded the best results with a cleanup efficiency of 93% and recovery of about 85%, representing an increase of toxin percentage by 13 fold. Expand
Variability in Toxin Profiles of the Mediterranean Ostreopsis cf. ovata and in Structural Features of the Produced Ovatoxins.
TLDR
Toxin content was mostly in the range 4-70 pg/cell with higher levels being found in strains from the Ligurian and South Adriatic Sea, and the new OVTX-l was detected in 36 strains. Expand
Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification.
TLDR
Investigation of a French O. ovata strain collected at Villefranche-sur-Mer during a bloom in 2011 confirmed the production of ovatoxins-a to -e and revealed the presence of a new ovat toxin analog, named ovatoxin-h, which is situated between C42 and C49. Expand
Harmful dinoflagellate Ostreopsis cf. ovata Fukuyo: detection of ovatoxins in field samples and cell immunolocalization using antipalytoxin antibodies.
TLDR
Since harmful dinoflagellates do not always produce toxins, the immunocytochemical localization of ovatoxins, although qualitative, can provide an early warning for toxic Ostreopsis cells before their massive diffusion and/or concentration in seafood. Expand
Toxin profile of Ostreopsis cf. ovata from Portuguese continental coast and Selvagens Islands (Madeira, Portugal).
TLDR
The present study reports for the first time the occurrence of several OVTX congeners and iso-PLTX in O. ovata from Portuguese waters, providing valuable information to characterize the risk of OV TXs-related outbreaks in Portugal. Expand
Structural elucidation of palytoxin analogs produced by the dinoflagellate Ostreopsis ovata IK2 strain by complementary use of positive and negative ion liquid chromatography/quadrupole time-of-flight mass spectrometry.
TLDR
The structures of ovatoxins produced by the IK2 strain of Ostreopsis ovata collected in Japan were elucidated using liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/QTOFMS) and a new insight into the spectral data is presented. Expand
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