Complex formation between the adenovirus DNA-binding protein and single-stranded poly(rA). Cooperativity and salt dependence.

@article{Kuil1989ComplexFB,
  title={Complex formation between the adenovirus DNA-binding protein and single-stranded poly(rA). Cooperativity and salt dependence.},
  author={Maxim Emile Kuil and Herbert van Amerongen and Peter C. van der Vliet and Rienk van Grondelle},
  journal={Biochemistry},
  year={1989},
  volume={28 25},
  pages={
          9795-800
        }
}
The complex formed between adenovirus DNA-binding protein (AdDBP) and poly(rA) was investigated with circular dichroism spectroscopy. The binding process was studied at a variety of salt concentrations, and the titration curves were analyzed according to the contiguous cooperative binding model given by McGhee and von Hippel [McGhee, J.D., & von Hippel, P.H. (1974) J. Mol. Biol. 86, 469-489]. The cooperativity factor omega of the binding process is low (omega approximately 20-30) and… CONTINUE READING
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