Completion of Tricin Biosynthesis Pathway in Rice: Cytochrome P450 75B4 Is a Unique Chrysoeriol 5′-Hydroxylase1

@article{Lam2015CompletionOT,
  title={Completion of Tricin Biosynthesis Pathway in Rice: Cytochrome P450 75B4 Is a Unique Chrysoeriol 5′-Hydroxylase1},
  author={Pui Ying Lam and Hongjiao Liu and Clive Lo},
  journal={Plant Physiology},
  year={2015},
  volume={168},
  pages={1527 - 1536}
}
Biosynthesis of tricin requires specific hydroxylation catalyzed by a phylogenetically distinct hydroxylase. Flavones are ubiquitously accumulated in land plants, but their biosynthesis in monocots remained largely elusive until recent years. Recently, we demonstrated that the rice (Oryza sativa) cytochrome P450 enzymes CYP93G1 and CYP93G2 channel flavanones en route to flavone O-linked conjugates and C-glycosides, respectively. In tricin, the 3′,5′-dimethoxyflavone nucleus is formed before O… Expand
Convergent recruitment of 5´-hydroxylase activities by CYP75B flavonoid B-ring hydroxylases for tricin biosynthesis in Medicago legumes.
TLDR
Investigation of a subgroup of cytochrome P450 (CYP) 75B subfamily flavonoid B-ring hydroxylases (FBHs) from two tricin-accumulating legumes revealed that these Medicago legumes had acquired thetricin pathway through molecular evolution of CYP75B FBHs subsequent to speciation from other non-tricin-coating legumes. Expand
Recruitment of specific flavonoid B-ring hydroxylases for two independent biosynthesis pathways of flavone-derived metabolites in grasses.
In rice (Oryza sativa), OsF2H and OsFNSII direct flavanones to independent pathways that form soluble flavone C-glycosides and tricin-type metabolites (both soluble and lignin-bound), respectively.Expand
Molecular and Biochemical Analysis of Two Rice Flavonoid 3’-Hydroxylase to Evaluate Their Roles in Flavonoid Biosynthesis in Rice Grain
TLDR
Gene expression analysis showed that CYP75B3, CYP 75B4, and most other flavonoid pathway genes were predominantly expressed in the developing seeds of black rice, but not in those of white and red rice, which is consistent with the pigmentation patterns of the seeds. Expand
OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls
TLDR
Cell wall structural analyses establish OsCAldOMT1 as a bifunctional O-methyltransferase predominantly involved in the two parallel metabolic pathways both dedicated to the biosynthesis of tricin-lignins in rice cell walls. Expand
Tricin Biosynthesis and Bioengineering
TLDR
This review summarizes the current understanding of tricin biosynthetic pathway in grasses andtricin-accumulating dicots and describes the characterized and potential enzymes involved in tricIn biosynthesis. Expand
Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3′- and 5′-O-Methyltransferases Involved in Lignin Biosynthesis
TLDR
It is suggested that CCoAOMT1 is involved in lignin biosynthesis at least in midribs, and the role of the flavonolignin unit derived from tricin in cell wall degradability is discussed. Expand
Title : Changes In Cell Wall Polymers And Degradability In Maize Mutants Lacking 3 ′-And 5 ′-O-Methyltransferases Involved In Lignin Biosynthesis Running head : Effects of COMT and CCoAOMT 1 knockout in maize
Caffeoyl Coenzyme A 3-O-Methyltransferase (CCoAOMT) and Caffeic acid-O-Methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G)Expand
Molecular and Functional Characterization of Oryza sativa Flavonol Synthase (OsFLS), a Bifunctional Dioxygenase.
TLDR
It is demonstrated that OsFLS is a bifunctional 2-ODD enzyme and functions in flavonol production in planta and was developmentally regulated during seed maturation. Expand
Evolutionary dynamic analyses on monocot flavonoid 3′-hydroxylase gene family reveal evidence of plant-environment interaction
TLDR
This study provided an evolutionary and protein structural explanation to the previously observed chrysoeriol-specific 5′-hydroxylation activity for CYP75B4 in rice, which may also be true for other Class II F3’Hs in monocots. Expand
Disrupting Flavone Synthase II Alters Lignin and Improves Biomass Digestibility1[OPEN]
TLDR
The tricin-deficient fnsII mutant was analyzed further and revealed to have enhanced enzymatic saccharification efficiency, suggesting that the cell wall recalcitrance of grass biomass may be reduced through the manipulation of the flavonoid monomer supply for lignification. Expand
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It is established that CYP93G1 is a key branch point enzyme channeling flavanones to the biosynthesis of tricin O-linked conjugates in rice and functional diversification of F2H and FNSII in the cytochrome P450 CYP 93G subfamily may represent a lineage-specific event leading to the prevalent cooccurrence of flavone C- and O- linked derivatives in grasses today. Expand
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