Complete reconstitution of conjugation and subsequent degradation of the tumor suppressor protein p53 by purified components of the ubiquitin proteolytic system.

@article{Shkedy1994CompleteRO,
  title={Complete reconstitution of conjugation and subsequent degradation of the tumor suppressor protein p53 by purified components of the ubiquitin proteolytic system.},
  author={D Shkedy and Hedva Gonen and Beatrice Bercovich and Aaron Ciechanover},
  journal={FEBS letters},
  year={1994},
  volume={348 2},
  pages={126-30}
}
The wild-type tumor suppressor protein p53 is a short-lived protein that plays important roles in regulation of cell cycle, differentiation, and survival. Mutations that inactivate or alter the tumor suppressor activity of the protein seem to be the most common genetic change in human cancer and are frequently associated with changes in its stability. The ubiquitin system has been implicated in the degradation of p53 both in vivo and in vitro. A mutant cell line that harbors a thermolabile… CONTINUE READING