Complete protection of islets against allorejection and autoimmunity by a simple barium-alginate membrane.

@article{DuvivierKali2001CompletePO,
  title={Complete protection of islets against allorejection and autoimmunity by a simple barium-alginate membrane.},
  author={Val{\'e}rie F Duvivier-Kali and Abdulkadir Omer and Richard J Parent and John J. O'neil and Gordon C. Weir},
  journal={Diabetes},
  year={2001},
  volume={50 8},
  pages={
          1698-705
        }
}
We describe a new technique for microencapsulation with high-mannuronic acid (high-M) alginate crosslinked with BaCl(2) without a traditional permselective component, which allows the production of biocompatible capsules that allow prolonged survival of syngeneic and allogeneic transplanted islets in diabetic BALB/c and NOD mice for >350 days. The normalization of the glycemia in the transplanted mice was associated with normal glucose profiles in response to intravenous glucose tolerance tests… 
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Survival of Microencapsulated Adult Pig Islets in Mice In Spite of an Antibody Response
TLDR
Alginate capsules can prolong the survival of adult pig islets transplanted into diabetic mice for up to 190 days, even in the presence of an antibody response.
Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression1
TLDR
Agarose microcapsules were able to completely protect NOD islet isografts from autoimmune destruction in the syngeneic islet transplantation model.
The effect of capsule composition in the reversal of hyperglycemia in diabetic mice transplanted with microencapsulated allogeneic islets.
TLDR
Investigation of how the biocompatibility of such capsules affects their function in vivo and their efficacy relative to naked islets after intraperitoneal transplantation to nude or immune competent mice found islets in high M alginate capsules can reverse hyperglycemia in nude and immune competent mouse.
Immunoisolation of pancreatic islets via thin-layer surface modification.
Intraportal transplantation of allogenic pancreatic islets encapsulated in barium alginate beads in diabetic rats.
TLDR
The aim of the present study was to test the hypothesis that the intraportal transplantation of allogenic islets encapsulated in small-sized barium alginate beads is safe and succeeds to induce normoglycemia in diabetic rats, and it is believed that the occlusion of small- and medium-sized portal venules by the alginates is responsible for this effect.
Biocompatibility and function of microencapsulated pancreatic islets.
Alginate microcapsules for pancreatic islet cell graft immunoprotection: struggle and progress towards the final cure for type 1 diabetes mellitus
  • R. Calafiore
  • Medicine, Biology
    Expert opinion on biological therapy
  • 2003
TLDR
Using neonatal porcine islets enveloped in AG-PLO microcapsules, hyperglycaemia has been corrected in several diabetic animal models and micro Capsules containing either human or, possibly, pig islets could be close to approval for Phase I human clinical trials.
Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques
TLDR
Transplantation of pancreatic islet cells encapsulated in alginate microspheres into the omental bursa of the peritoneal cavity of NHPs significantly reduces FBRs and extends the longevity of the cells.
Avoiding Immunosuppression for Islet Transplantation: Use of Protective Biomaterials
TLDR
This chapter reviews the variety of technologies used to protect transplanted islets against toxicity of immunosuppression, immune rejection, and inflammatory response and evaluates the mechanisms of these technologies and their progress in solving the challenges to islet transplantation.
Survival of Microencapsulated Islets at 400 Days Posttransplantation in the Omental Pouch of NOD Mice
TLDR
The long-term durability of agarose microencapsulated islets against autoimmunity in a syngeneic islet transplantation model in NOD mice was evaluated and it was demonstrated that the microcapsules successfully protected the islets from cellular immunity.
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References

SHOWING 1-10 OF 31 REFERENCES
Prolonged Reversal of Diabetic State in NOD Mice by Xenografts of Microencapsulated Rat Islets
TLDR
Results clearly indicate that encapsulation of pancreatic islets in the improved capsules can effectively prolong xenograft survival without immunosuppression in an animal model that mimics human type I diabetes mellitus.
Porcine pancreatic islets: isolation, microencapsulation, and xenotransplantation.
TLDR
In vitro studies demonstrated that the isolated islets secreted insulin in response to glucose and 3-isobutyl-L-methylxanthine (IBMX) challenge for at least 4 weeks, and perifusion studies showed that the kinetics of insulin release from the encapsulated islets was similar to that exhibited by free islets.
Normalization of diabetes in spontaneously diabetic cynomologus monkeys by xenografts of microencapsulated porcine islets without immunosuppression.
TLDR
Examination of internal organs of two of the animals involved in the transplantation studies for the duration of 2 yr revealed no untoward effect of the extended presence of the microcapsules.
Strategy for developing microbeads applicable to islet xenotransplantation into a spontaneous diabetic NOD mouse.
TLDR
Novel microbeads are proposed that can realize long-term BAP function in NOD mice and are believed to be promising for producing semipermeable membranes that enable xenotransplantation of islets in spontaneous diabetes mellitus.
Insulin secretion in streptozotocin-diabetic rats transplanted with immunoisolated islets.
TLDR
It was found that rats transplanted with 1500 microencapsulated islets similarly were normoglycemic for 3 weeks and that the plasma insulin response to glucose infusion at day 10 was normal, andMicroencapsulation is superior to the use of diffusion chamber as the immunoisolation technique for islet transplantation.
Prolonged survival of transplanted islets of Langerhans encapsulated in a biocompatible membrane.
Correction of diabetic nod mice with insulinomas implanted within Baxter immunoisolation devices
TLDR
The results with allogeneic mouseβ-cells suggest the possiblity that immortalized human β-cells could be an effective source of tissue to correct diabetes in IDDM patients without the use of immunosuppression.
PROTECTION OF ISLETS OF LANGERHANS FROM INTERLEUKIN‐1 TOXICITY BY ARTIFICIAL MEMBRANES
TLDR
The present in vitro study showed that the encapsulated rat islets are protected from high doses of IL-1 inside a hollow fiber membrane with a cutoff of 50,000 D.
C-Peptide responses after meal challenge in mice transplanted with microencapsulated rat islets
TLDR
The delivery of C peptide and presumably the accompanying insulin are delayed by restrictions of the capsules and the peritoneal location, but this delay in reaching peripheral target organs does not prevent microencapsulated grafts from efficiently clearing glucose after a meal.
Xenotransplantation of cells using biodegradable microcapsules.
TLDR
A hydrogel-based microcapsule that breaks down at a rate that can be adjusted to correspond to the functional longevity of the encapsulated cells, which allows breakdown and absorption of implants when the cells die or become functionally inactive is described.
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