Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi.

@article{Hashimoto1993CompleteNS,
  title={Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi.},
  author={Y. Hashimoto and N. Li and H. Yokoyama and T. Ezaki},
  journal={Journal of bacteriology},
  year={1993},
  volume={175 14},
  pages={
          4456-65
        }
}
Plasmid pGBM124, which contains a 14-kb Salmonella typhi chromosomal DNA fragment capable of producing the Vi antigen in Escherichia coli HB101 and ViaB-deleted S. typhi GIFU 10007-3, was studied. We determined the complete nucleotide sequence of this fragment and found 11 open reading frames. Mutagenesis, subcloning, and complementation analysis showed that three genes (vipA, vipB, and vipC) are involved in biosynthesis of the Vi polysaccharide. The putative primary amino acid sequence… Expand
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