Complete mutagenesis of the HIV-1 protease

@article{Loeb1989CompleteMO,
  title={Complete mutagenesis of the HIV-1 protease},
  author={D. Loeb and R. Swanstrom and L. Everitt and M. Manchester and S. Stamper and C. Hutchison},
  journal={Nature},
  year={1989},
  volume={340},
  pages={397-400}
}
RETROVIRUSES encode a protease which needs to be active for the production of infectious virions. A disabling mutation in the protease results in the production of non-infectious virus particles and examination of proteins from these mutant virions reveals unprocessed Gag and Gag-Pol precursor proteins, the substrates of the viral protease1-3. Each amino acid of the HIV-1 protease was individually mutated using a simple mutagenesis procedure which is capable of introducing and identifying… Expand
Analysis of temperature-sensitive mutants of the HIV-1 protease.
Expression systems for retroviral proteases.
HIV-1 protease dimer interface mutations that compensate for viral reverse transcriptase instability in infectious virions.
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