Complete inhibition of the Pdr5p multidrug efflux pump ATPase activity by its transport substrate clotrimazole suggests that GTP as well as ATP may be used as an energy source.

Abstract

The yeast Pdr5p transporter is a 160 kDa protein that effluxes a large variety of xenobiotic compounds. In this study, we characterize its ATPase activity and demonstrate that it has biochemical features reminiscent of those of other ATP-binding cassette multidrug transporters: a relatively high Km for ATP (1.9 mM), inhibition by orthovanadate, and the… (More)

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@article{Golin2007CompleteIO, title={Complete inhibition of the Pdr5p multidrug efflux pump ATPase activity by its transport substrate clotrimazole suggests that GTP as well as ATP may be used as an energy source.}, author={John E. Golin and Zachary N Kon and Chung-Pu Wu and Justin Martello and Leanne Hanson and Sherry L. Supernavage and Suresh V Ambudkar and Zuben E. Sauna}, journal={Biochemistry}, year={2007}, volume={46 45}, pages={13109-19} }