Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans

@article{Prust2005CompleteGS,
  title={Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans},
  author={Christina Prust and M. Hoffmeister and Heiko Liesegang and Arnim Wiezer and Wolfgang Florian Fricke and Armin Ehrenreich and Gerhard Gottschalk and Uwe Deppenmeier},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={195-200}
}
Gluconobacter oxydans is unsurpassed by other organisms in its ability to incompletely oxidize a great variety of carbohydrates, alcohols and related compounds. Furthermore, the organism is used for several biotechnological processes, such as vitamin C production. To further our understanding of its overall metabolism, we sequenced the complete genome of G. oxydans 621H. The chromosome consists of 2,702,173 base pairs and contains 2,432 open reading frames. In addition, five plasmids were… 

Physiology of Acetic Acid Bacteria in Light of the Genome Sequence of Gluconobacter oxydans

Based on the genome sequence of Gluconobacteroxydans, light is shed on the central carbon metabolism, the composition of the respiratory chain and the analysis of uncharacterized oxidoreductases, which are of major importance in the process of incomplete oxidation.

Global mRNA decay and 23S rRNA fragmentation in Gluconobacter oxydans 621H

The very short mRNA half-lives of the H+-ATP synthase, which is likely responsible for the ATP-proton motive force interconversion in G. oxydans under many or most conditions, is notably in contrast to mRNA decay data from other bacteria.

An easy cloning and expression vector system for Gluconobacter oxydans.

Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans.

Strain development of Gluconobacter oxydans: Complementation of non-functional metabolic pathways and increase of carbon flux

The obligatory aerobic acetic acid bacterium Gluconobacter oxydans rapidly oxidizes diverse carbon sources regioand stereoselectively in the periplasm to increase the cell yield on glucose and serve as suitable host for further metabolic engineering addressing bottlenecks in pyruvate and acetylCoA oxidation.

Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H

Gluconobacter oxydans shows a number of exceptional characteristics, like the biphasic growth on glucose and the incomplete oxidation of glucose to gluconate (phase I, exponential growth,) and

Identification of Gradient Promoters of Gluconobacter oxydans and Their Applications in the Biosynthesis of 2-Keto-L-Gulonic Acid

The low activity of SDH was found to hamper the high yield of 2-KLG, and enhancing the expression ofSDH was achieved by screening the suitable promoters based on RNA sequencing data, and a series of gradient promoters were obtained, including two strong shuttle promoters and six strongest promoters were used to overexpress SDH in G. oxydans WSH-003.

Characterization of membrane-bound dehydrogenases of Gluconobacter oxydans 621H using a new system for their functional expression

The constitutive promoters of the alcohol dehydrogenase and the glucose-repressed promoter of the inositol dehydration were used to construct a shuttle vector system for the fully functional expression of mDHs in the multi-deletion strain G. oxydans BP.

The Genus Gluconobacter Oxydans: Comprehensive Overview of Biochemistry and Biotechnological Applications

This review aims to give an overview of the myriad of applications for Gluconobacter, with a special focus on some recent developments.
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