Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses.

Abstract

In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.

DOI: 10.1016/j.virol.2016.11.006

Cite this paper

@article{Absaln2017CompleteGS, title={Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses.}, author={{\'A}ngel E. Absal{\'o}n and Andr{\'e}s Morales-Garz{\'o}n and Pedro F Vera-Hern{\'a}ndez and Diana Ver{\'o}nica Cort{\'e}s-Espinosa and Sara M Uribe-Ochoa and Laura J. Garc{\'i}a and Eduardo Lucio-Decanini}, journal={Virology}, year={2017}, volume={501}, pages={63-69} }