Complete genome sequence of a new bipartite begomovirus infecting cotton in the Republic of Benin in West Africa

@article{Leke2016CompleteGS,
  title={Complete genome sequence of a new bipartite begomovirus infecting cotton in the Republic of Benin in West Africa},
  author={Walter Nkeabeng Leke and Behnam Khatabi and Djana Babatima Mignouna and Judith K. Brown and Vincent N. Fondong},
  journal={Archives of Virology},
  year={2016},
  volume={161},
  pages={2329-2333}
}
Here, we report the complete genome sequence of a novel bipartite begomovirus isolated from cotton plants (Gossypium raimondii, Malvaceae) exhibiting light yellow mosaic symptoms. The genome sequence was determined by Illumina DNA sequencing and confirmed by Sanger sequencing of RCA-enriched, cloned circular genomic components. The DNA-A and DNA-B components were each ~2.7 kb in size, and their genome arrangement was characteristic of other Old World bipartite begomoviruses. While the DNA-A… Expand
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References

SHOWING 1-10 OF 24 REFERENCES
Complete nucleotide sequences of two new begomoviruses infecting the wild malvaceous plant Melochia sp. in Brazil
TLDR
The complete genome sequences of two new begomoviruses from Melochia sp. Expand
Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan.
TLDR
The data suggest that a nanovirus-like DNA has become whitefly-transmissible as a result of its association with a geminivirus and that cotton leaf curl disease may result from a mutually dependent relationship that has developed between members of two distinct DNA virus families that share a similar replication strategy. Expand
Cotton leaf curl Gezira virus-satellite DNAs represent a divergent, geographically isolated Nile Basin lineage: predictive identification of a satDNA REP-binding motif.
TLDR
A previously unknown lineage of single-stranded DNA satellite (satDNA) molecules, which are associated with CLCuGV, and are required for development of characteristic disease symptoms in Sudan, form a new satDNA lineage comprising only satDNAs from the Upper and Lower Nile Basins. Expand
A Melting Pot of Old World Begomoviruses and Their Satellites Infecting a Collection of Gossypium Species in Pakistan
TLDR
An exhaustive analysis of the diversity of begomoviruses and their satellites occurring in 15 Gossypium species that are maintained in an orchard in the vicinity of Multan has been conducted, finding a much greater diversity of alphasatellites in the non-cultivated cotton species than in G. hirsutum. Expand
Revision of Begomovirus taxonomy based on pairwise sequence comparisons
TLDR
Revised guidelines for the classification and nomenclature of begomoviruses are proposed and genome-wide pairwise identities of 91 % and 94 % are proposed as the demarcation threshold for begomviruses belonging to different species and strains, respectively. Expand
Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda.
TLDR
Analysis of naturally infected cassava plants showed various assortments of DNA-A and DNA-B of the Ugandan viruses, suggesting the occurrence of natural inter- and intraspecies pseudorecombinations and a pattern of cassava mosaic disease (CMD) more complex than previously reported. Expand
Light-dependent segregation of begomoviruses in Asystasia gangetica leaves.
TLDR
The relationship between leaf symptoms and virus distribution under different light regimes was investigated, and showed for the first time an unusual segregation of symptoms and viruses, either within a single plant, or even within a leaf. Expand
Viral Metagenomics: Analysis of Begomoviruses by Illumina High-Throughput Sequencing
TLDR
The use of NGS approaches, together with advances in software development, make possible deep sequence coverage at a lower cost; the approach described herein will streamline the exploration of begomovirus diversity and population structure from naturally infected plants, irrespective of viral abundance. Expand
A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue.
TLDR
This method should be extremely useful for the study of any circular DNA plant viruses with genome component lengths smaller than the maximum size amplifiable by RCA. Expand
Cotton leaf curl disease - an emerging threat to cotton production worldwide.
TLDR
Recent advances made in understanding the molecular biology of the components of the disease complex, their interactions with host plants, as well as efforts being made to control CLCuD are outlined. Expand
...
1
2
3
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