Complete complementary DNA sequence of the third component of complement of lamprey. Implication for the evolution of thioester containing proteins.

@article{Nonaka1992CompleteCD,
  title={Complete complementary DNA sequence of the third component of complement of lamprey. Implication for the evolution of thioester containing proteins.},
  author={Mayumi Nonaka and Minoru Takahashi},
  journal={Journal of immunology},
  year={1992},
  volume={148 10},
  pages={3290-5}
}
Lamprey liver mRNA sequences were amplified by reverse transcriptase-polymerase chain reaction using primers synthesized according to the amino acid sequences at the thioester region common to the mammalian C3, C4, and alpha 2-macroglobulin (alpha 2M). Two different cDNA species were identified that showed a close similarity to the mammalian C3 or alpha 2M sequences, respectively. Using the C3-like sequence as a probe, two overlapping cDNA clones were isolated from the lambda ZAP library, which… CONTINUE READING