Complete biosynthetic pathway of alazopeptin, a tripeptide con-sisting of two molecules of 6-diazo-5-oxo-L-norleucine and one molecule of alanine.

@article{Kawai2021CompleteBP,
  title={Complete biosynthetic pathway of alazopeptin, a tripeptide con-sisting of two molecules of 6-diazo-5-oxo-L-norleucine and one molecule of alanine.},
  author={Seiji Kawai and Yuko Sugaya and Ryota Hagihara and Hiroya Tomita and Yohei Katsuyama and Yasuo Ohnishi},
  journal={Angewandte Chemie},
  year={2021}
}
DON (6-Diazo-5-oxo-L-norleucine), a diazo-containing amino acid, has been studied for more than 60 years as a potent antitumor agent, but its biosynthesis has not been elucidated. Here we reveal the complete biosynthetic pathway of alazopeptin, the tripeptide Ala-DON-DON which has antitumor activity, by gene inactivation and in vitro analysis of recombinant enzymes. We also established heterologous production of N-acetyl-DON in Streptomyces albus. DON is synthesized from lysine by three enzymes… 
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