Complete activation of Bax by a single site mutation

  title={Complete activation of Bax by a single site mutation},
  author={Huiqing Zhou and Qi Ming Hou and Jakob Lerche Hansen and Y T Hsu},
Bax translocation from the cytosol to mitochondria culminates a key step by which this protein mediates cell death. Here, we identified two amino acids, L70 and D71, within the BH3 domain of Bax that play a critical role in regulating Bax's cytosolic vs mitochondrial distribution. Individual substitution of these amino acids with alanine resulted in Bax conformational change, oligomerization, localization to mitochondria and cell death. Further mutational analysis indicated that L70 interacts… CONTINUE READING

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Apoptosis is triggered when prosurvival Bcl-2 proteins cannot restrain Bax.

Proceedings of the National Academy of Sciences of the United States of America • 2008
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