Complete Replication of Hepatitis C Virus in Cell Culture

@article{Lindenbach2005CompleteRO,
  title={Complete Replication of Hepatitis C Virus in Cell Culture},
  author={Brett Lindenbach and Matthew J Evans and Andrew J. Syder and Benno Wölk and Timothy L. Tellinghuisen and Christopher C. Liu and Toshiaki Maruyama and Richard O. Hynes and Dennis R. Burton and Jane A. McKeating and Charles M. Rice},
  journal={Science},
  year={2005},
  volume={309},
  pages={623 - 626}
}
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 105 infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral… 
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This work has identified an HCV isolate that replicates to very high levels in a human hepatoma cell line and provides new possibilities for molecular studies of the HCV life cycle and the development of novel antiviral concepts.
Generation and quantitation of infectious hepatitis C virus derived from cell culture (HCVcc).
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  • 2010
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The assays in this unit are based on protocols designed for Huh-7-derived cell lines that allow robust replication of HCV and are permissive to infection, important for the implementation of drug discovery efforts relative to the entire infectious virus life cycle.
On the history of hepatitis C virus cell culture systems.
TLDR
The initial difficulties to achieve HCV replication in cell culture are described, finally leading to the establishment of subgenomic replicons and the infectious virus model (HCVcc).
[Production of infectious hepatitis C virus in cell culture].
TLDR
The JFH-1 strain from a patient with fulminant hepatitis was isolated, and the JEH-1 subgenomic replicon could replicate efficiently in culture cell without adaptive mutation, and this system provides a powerful tool for studying the viral life cycle and constructing anti-viral strategies.
A novel virus culture system for hepatitis C virus
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A JFH-1 subgenomic replicon that replicated efficiently in cultured cells without adaptive mutations and secreted viral particles were infectious for cultured cells and in a chimpanzee.
Cell culture and infection system for hepatitis C virus
TLDR
This protocol describes how to use the JFH-1 viral culture system, which provides a powerful tool for studying viral life cycle and for the construction of antiviral strategies and the development of effective vaccines.
Hepatitis C virus molecular clones and their replication capacity in vivo and in cell culture.
TLDR
This review will summarize the development and current state of molecular HCV clones and discuss the prospects and implications of the most recent achievements.
Molecular biology of hepatitis C virus
TLDR
The development of reverse genetics-based experimental tools in HCV research can bring a greater understanding of the viral life cycle and pathogenesis of HCV-induced diseases.
Construction of the Vero cell culture system that can produce infectious HCV particles
TLDR
A HCV (1b) full-length cDNA clone that replicates and produces integrated and infectious virus particles in cultured Vero cells is used, establishing a powerful framework for studying the virus life cycle and developing vaccine research.
Entry and replication of recombinant hepatitis C viruses in cell culture.
TLDR
The present review aims at recapitulating the different viral and cellular systems modellingHCV entry and replication, and in particular at gathering the tools available to dissect the HCV entry pathway.
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