Complete Replication of Hepatitis C Virus in Cell Culture

@article{Lindenbach2005CompleteRO,
  title={Complete Replication of Hepatitis C Virus in Cell Culture},
  author={Brett Lindenbach and Matthew J Evans and Andrew J. Syder and Benno Wölk and Timothy L. Tellinghuisen and Christopher C. Liu and Toshiaki Maruyama and Richard O. Hynes and Dennis R. Burton and Jane A. McKeating and Charles M. Rice},
  journal={Science},
  year={2005},
  volume={309},
  pages={623 - 626}
}
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 105 infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral… 
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The initial difficulties to achieve HCV replication in cell culture are described, finally leading to the establishment of subgenomic replicons and the infectious virus model (HCVcc).
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A JFH-1 subgenomic replicon that replicated efficiently in cultured cells without adaptive mutations and secreted viral particles were infectious for cultured cells and in a chimpanzee.
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This protocol describes how to use the JFH-1 viral culture system, which provides a powerful tool for studying viral life cycle and for the construction of antiviral strategies and the development of effective vaccines.
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This review will summarize the development and current state of molecular HCV clones and discuss the prospects and implications of the most recent achievements.
Molecular biology of hepatitis C virus
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The development of reverse genetics-based experimental tools in HCV research can bring a greater understanding of the viral life cycle and pathogenesis of HCV-induced diseases.
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A HCV (1b) full-length cDNA clone that replicates and produces integrated and infectious virus particles in cultured Vero cells is used, establishing a powerful framework for studying the virus life cycle and developing vaccine research.
Entry and replication of recombinant hepatitis C viruses in cell culture.
TLDR
The present review aims at recapitulating the different viral and cellular systems modellingHCV entry and replication, and in particular at gathering the tools available to dissect the HCV entry pathway.
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