Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

@article{Gibson2008CompleteCS,
  title={Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome},
  author={D. G. Gibson and G. A. Benders and C. Andrews-Pfannkoch and E. Denisova and Holly Baden-Tillson and Jayshree Zaveri and T. Stockwell and Anushka Brownley and D. Thomas and Mikkel A. Algire and C. Merryman and L. Young and V. Noskov and J. Glass and J. Venter and C. Hutchison and H. Smith},
  journal={Science},
  year={2008},
  volume={319},
  pages={1215 - 1220}
}
We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted “watermarks” at intergenic sites known to tolerate transposon insertions. Overlapping “cassettes” of 5 to 7 kilobases (kb), assembled from chemically synthesized… Expand
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