Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

@article{Gibson2008CompleteCS,
  title={Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome},
  author={Daniel G. Gibson and Gwynedd A. Benders and Cynthia Andrews-Pfannkoch and Evgeniya A. Denisova and Holly Baden-Tillson and Jayshree Zaveri and Timothy B. Stockwell and Anushka Brownley and David W. Thomas and Mikkel A. Algire and Chuck Merryman and Lei Young and V. N. Noskov and John I. Glass and J. Craig Venter and Clyde A. Hutchison and Hamilton O. Smith},
  journal={Science},
  year={2008},
  volume={319},
  pages={1215 - 1220}
}
  • Daniel G. Gibson, Gwynedd A. Benders, +14 authors Hamilton O. Smith
  • Published 2008
  • Medicine, Biology
  • Science
  • We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted “watermarks” at intergenic sites known to tolerate transposon insertions. Overlapping “cassettes” of 5 to 7 kilobases (kb), assembled from chemically synthesized… CONTINUE READING

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