Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage.

@article{Balsalobre1996ComplementationOT,
  title={Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage.},
  author={Carlos Balsalobre and Antonio Ju{\'a}rez and Cristina Madrid and Mercedes Mouri{\~n}o and Antoni Prenafeta and F J Mu{\~n}oa},
  journal={Microbiology},
  year={1996},
  volume={142 ( Pt 7)},
  pages={
          1841-6
        }
}
The Hha protein from Escherichia coli is highly similar (82%) to the YmoA protein from Yersinia enterocolitica. Both are members of a new class of proteins that modulates gene expression, probably by influencing DNA topology. In this paper, complementation of the hha mutation in E. coli by the ymoA gene from Y. enterocolitica has been studied. We show that the ymoA gene complements one of the phenotypic properties of hha mutants (high level of haemolysin production when they carry the… 
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Role of plasmid- and chromosomally encoded Hha proteins in modulation of gene expression in E. coli O157:H7.
Alterations in Protein Expression Caused by thehha Mutation in Escherichia coli: Influence of Growth Medium Osmolarity
TLDR
Study of the effect of the hha mutation on the overall protein pattern of Escherichia coli cells by two-dimensional polyacrylamide gel electrophoresis suggested that at least some of the pleiotropic effects of the Hha mutation could be caused by its effect on the catabolite repression system.
Efficient expression of the α-haemolysin determinant in the uropathogenic Escherichia coli strain 536 requires the leuX-encoded tRNA5Leu
TLDR
It is demonstrated that the tRNA5Leu affects the expression of the α-haemolysin determinant at different levels in a regulatory cascade, and imply that, in addition to Hha, at least one further, as yet unidentified, regulatory factor must be involved in the regulation of hly transcription in the uropathogenic E. coli strain 536.
Expression of the hemolysin operon in Escherichia coli is modulated by a nucleoid-protein complex that includes the proteins Hha and H-NS
TLDR
DNA-binding studies suggest that in vivo H-NS and Hha form a nucleoid-protein complex that accounts for the thermo-osmotic regulation of the hemolysin operon in E. coli.
The Hha protein as a modulator of expression of virulence factors in Escherichia coli
TLDR
Hha derivatives from both a clinical uropathogenic Escherichia coli isolate and a wild E. coli strain causing bovine diarrhea were constructed and the effect of the hha allele on the expression of the different virulence factors exhibited by these strains was analyzed.
YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H‐NS and StpA
TLDR
The identification of the Hha paralogue in E. coli, the YdgT protein, which appears to fulfil some of the functions reported for StpA as H‐NS paralogue: Yd gT is overexpressed in hha mutants and can compensate, at least partially, some ofThe hha‐induced phenotypes, and protein cross‐linking studies showed that YdGT/H‐NS heteromeric complexes are generated within the bacterial cell.
Role of the Hha/YmoA family of proteins in the thermoregulation of the expression of virulence factors.
TLDR
The chromatin-associated protein H-NS has been shown to play a role in the thermomodulation of virulence factor expression, and the presence of genes coding for homologues of both proteins on some conjugative plasmids and their relation to thermoregulation suggests that this complex could also play arole in the regulation of plasmid transfer.
Hha Is a Negative Modulator of Transcription ofhilA, the Salmonella enterica Serovar Typhimurium Invasion Gene Transcriptional Activator
TLDR
Introduction of plasmid-encoded hha into strain SL1344 results in attenuation of invasion using in vitro and in vivo assays, and purified Hha protein was found to bind to a hilA DNA promoter fragment, suggesting that the regulatory activity of the Hhaprotein occurs at the hilB promoter.
Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin
TLDR
A model is presented to explain the temperature-modulated repression of the hly operon, based on the experiments reported here and other, preexisting data.
Calcium-Regulated Type III Secretion of Yop Proteins by an Escherichia coli hha Mutant Carrying a Yersinia pestis pCD1 Virulence Plasmid
TLDR
A series of four large deletions that removed a total of ca.
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