Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris.

Abstract

A PyrF- mutant of Escherichia coli (SK1108, pyrF::Tn5 Kanr) was complemented with the Desulfovibrio vulgaris (Hildenborough) structural gene for orotidine-5'-phosphate decarboxylase (EC 4.1.1.23). Either orientation of a 1.6-kilobase-pair D. vulgaris DNA fragment (pLP3B or pLP3A) complemented the PyrF- strain suggesting that the D. vulgaris pyrF promoter was functional. The apparent product of the D. vulgaris pyrF gene was a single 26-kilodalton polypeptide. These results demonstrate the utility of E. coli cloning systems in studying metabolic and energetic pathways in sulfate-reducing bacteria.

Cite this paper

@article{Li1986ComplementationOA, title={Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris.}, author={Cui Li and Harry D. Peck and Alan E Przybyla}, journal={Journal of bacteriology}, year={1986}, volume={165 2}, pages={644-6} }