Complementation in vitro between guaB mutants of Escherichia coli K12.

@article{Gilbert1980ComplementationIV,
  title={Complementation in vitro between guaB mutants of Escherichia coli K12.},
  author={Harry J. Gilbert and William T. Drabble},
  journal={Journal of general microbiology},
  year={1980},
  volume={117 1},
  pages={
          33-45
        }
}
Guanine auxotrophs of Escherichia coli were isolated following mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine or ethyl methanesulphonate. The mutants were classified according to growth properties and absence of IMP dehydrogenase or GMP synthetase activity. Mutations in guaB (IMP dehydrogenase-less) were analysed by reversion and suppression tests; all were of the base substitution missense type except for one possible frameshift and one polar nonsense mutation. GuaB mutants were examined… CONTINUE READING