Complementary retrieval of 16S rRNA gene sequences using broad-range primers with inosine at the 3'-terminus: implications for the study of microbial diversity.

@article{Brands2010ComplementaryRO,
  title={Complementary retrieval of 16S rRNA gene sequences using broad-range primers with inosine at the 3'-terminus: implications for the study of microbial diversity.},
  author={Berit Brands and M E Vianna and Ilse Seyfarth and Georg Conrads and H. -P. Horz},
  journal={FEMS microbiology ecology},
  year={2010},
  volume={71 1},
  pages={157-67}
}
We evaluated the impact of the base analogue inosine substituted at the 3'-terminus of broad-range 16S rRNA gene primers on the recovery of microbial diversity using terminal restriction fragment length polymorphism and clonal analysis. Oral plaque biofilms from 10 individuals were tested with modified and unmodified primer pairs. Besides a core overlap of shared terminal restriction fragments (T-RFs), each primer system provided unique information on the occurrence of T-RFs, with a higher… CONTINUE READING
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