Complementary deoxyribonucleic acid cloning and characterization of mSP-10: the mouse homologue of human acrosomal protein SP-10.

@article{Reddi1995ComplementaryDA,
  title={Complementary deoxyribonucleic acid cloning and characterization of mSP-10: the mouse homologue of human acrosomal protein SP-10.},
  author={Prabhakara P Reddi and Soren Naaby-Hansen and I Aguolnik and Jeng Y Tsai and Lee M. Silver and Charles J. Flickinger and John C. Herr},
  journal={Biology of reproduction},
  year={1995},
  volume={53 4},
  pages={873-81}
}
Complementary DNA encoding the putative mouse homologue for human acrosomal protein SP-10, a candidate contraceptive vaccinogen, was cloned and sequenced. The entire open reading frame (amino acids 18 to 261) of the mouse SP-10 (mSP-10), with the exception of the signal peptide (amino acids 1 to 17), was placed under the influence of inducible T7 RNA polymerase/promoter system to overproduce recombinant protein (re-mSP-10) in Escherichia coli. A six-histidine tag, which was coexpressed at the… CONTINUE READING

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