Complementary DNA synthesis in situ: methods and applications.

  title={Complementary DNA synthesis in situ: methods and applications.},
  author={James H. Eberwine and Charlotte A. Spencer and Kevin Y. Miyashiro and Scott A. Mackler and Richard Finnell},
  journal={Methods in enzymology},
In situ transcription is the synthesis of cDNA within cells. This chapter has illustrated some of the application of IST to the study of gene expression in complex cell environments. While the importance of transcription in modulating cellular activity has been long appreciated, the role of translational control mechanisms in regulating central nervous system functioning is just beginning to be recognized. Previous limitations in the availability of tissue have made it difficult to construct… CONTINUE READING