Complementary DNA sequencing: expressed sequence tags and human genome project

@article{Adams1991ComplementaryDS,
  title={Complementary DNA sequencing: expressed sequence tags and human genome project},
  author={M. D. Adams and J. Kelley and JD Gocayne and M. Dubnick and MH Polymeropoulos and H. Xiao and CR Merril and A. Wu and B. Olde and RF Moreno and al. et},
  journal={Science},
  year={1991},
  volume={252},
  pages={1651 - 1656}
}
  • M.D. Adams, J. Kelley, +8 authors al. et
  • Published 1991
  • Biology, Medicine
  • Science
  • Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila… CONTINUE READING
    2,142 Citations

    Paper Mentions

    Sequence identification of 2,375 human brain genes
    • 691
    Long human-mouse sequence alignments reveal novel regulatory elements: a reason to sequence the mouse genome.
    • 334
    • PDF
    Large-scale concatenation cDNA sequencing.
    • 51
    • PDF
    2006 expressed-sequence tags derived from human chromosome 7-enriched cDNA libraries.
    • 15
    • PDF
    Identification of chromosome-specific sequence-tagged sites by Alu-PCR.
    • 3
    Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)
    • 20
    • PDF
    Single nucleotide polymorphism hunting in cyberspace
    • 60

    References

    SHOWING 1-10 OF 56 REFERENCES
    An 'equalized cDNA library' by the reassociation of short double-stranded cDNAs.
    • M. Ko
    • Medicine, Biology
    • Nucleic acids research
    • 1990
    • 118
    Construction of a uniform-abundance (normalized) cDNA library.
    • 154
    • PDF
    Mapping and sequencing the human genome.
    • V. A. Mckusick
    • Biology, Medicine
    • The New England journal of medicine
    • 1989
    • 167
    mRNA in the mammalian central nervous system.
    • J. Sutcliffe
    • Biology, Medicine
    • Annual review of neuroscience
    • 1988
    • 127