Complementary DNA sequencing: expressed sequence tags and human genome project

  title={Complementary DNA sequencing: expressed sequence tags and human genome project},
  author={M. D. Adams and J. Kelley and JD Gocayne and M. Dubnick and MH Polymeropoulos and H. Xiao and CR Merril and A. Wu and B. Olde and RF Moreno and al. et},
  pages={1651 - 1656}
  • M.D. Adams, J. Kelley, +8 authors al. et
  • Published 1991
  • Biology, Medicine
  • Science
  • Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila… CONTINUE READING
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