Complementary DNA libraries

@article{Ying2004ComplementaryDL,
  title={Complementary DNA libraries},
  author={S Y Ying},
  journal={Molecular Biotechnology},
  year={2004},
  volume={27},
  pages={245-252}
}
  • S. Ying
  • Published 1 July 2004
  • Biology, Medicine
  • Molecular Biotechnology
The generation of complete and full-length cDNA libraries for potential functional assays of specific gene sequences is essential for most molecules in biotechnology and biomedical research. The field of cDNA library generation has changed rapidly in the past 10 yr. This review presents an overview of the method available for the basic information of generating cDNA libraries, including the definition of the cDNA library, different kinds of cDNA libraries, difference between methods for cDNA… Expand
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References

SHOWING 1-10 OF 26 REFERENCES
Construction of a uniform-abundance (normalized) cDNA library.
TLDR
Libraries of these fragments are suitable for cDNA subtraction, screening, or selection by hybridization and make it possible to detect and analyze cDNA corresponding to species of mRNA present at a low level in a small fraction of the cells in a complex tissue. Expand
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
  • M. Frohman, M. Dush, G. Martin
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
TLDR
The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases. Expand
The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNA
TLDR
The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence. Expand
In vivo analysis of cancerous gene expression by RNA-polymerase chain reaction.
TLDR
The amplified mRNA libraries were shown to possess high fidelity, purity, specificity and reproducibility for in vivo analyses of cancerous gene expression in human prostate cancers. Expand
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
TLDR
A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer. Expand
cDNA detection and analysis.
TLDR
In the continuing search for a full-length cDNA cloning method, there is no clear winner, but advances in serial analysis of gene expression provide a quantitative, if expensive, alternative to these methods. Expand
One-sided polymerase chain reaction: the amplification of cDNA.
  • O. Ohara, R. Dorit, W. Gilbert
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1989
TLDR
A rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs, which underscores the conservative nature of the tropomyosin molecule and support the notion of a constrained heptapeptide unit as the fundamental structural motif of tropomyOSin. Expand
Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
TLDR
The ability of lambda ZAP to serve as a vector for the construction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA's using rabbit IgG anti-glucocerbosidase antibodies. Expand
Rat insulin genes: construction of plasmids containing the coding sequences.
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing theExpand
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
TLDR
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Expand
...
1
2
3
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