Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA.

@article{Stieger1991CompetitivePC,
  title={Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA.},
  author={Martin Stieger and Corinne D{\'e}molli{\`e}re and Ludwina Ahlborn-Laake and Johannes Gerardus Cornelius Mous},
  journal={Journal of virological methods},
  year={1991},
  volume={34 2},
  pages={
          149-60
        }
}
A quantitative PCR assay for the detection of HIV-1 nucleic acids is described. The assay is based on a competitive internal standard nucleic acid which can be discriminated from target sequences by the presence of a new restriction enzyme site. The method was used to quantitate plasmid molecules containing HIV-1 sequences, HIV-1 DNA and HIV-1 RNA purified from HIV-1-infected tissue culture cells as well as HIV-1 DNA present in the peripheral blood mononuclear cells of an AIDS patient. The… CONTINUE READING
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