Competitive immunoassay by hot electron-induced electrochemiluminescence detection and using a semiautomatic electrochemiluminometer

Abstract

A homogeneous immunoassay of T4 was developed using a semiautomatic electrochemiluminometer modified from a commercially available fluorometer. In addition, from the same analyte panel an immunometric immunoassay of TSH at similar disposable oxide-covered aluminum rake electrodes was studied using this instrument both on homogeneous and heterogeneous basis. Detection was based on hot electron-induced cathodic electrochemiluminescence utilizing a commercially available Tb(III) chelate label. The assays were reasonably sensitive and comparison was made with other older methods. Thus, it is possible to develop both non-competitive and competitive immunoassays based on detection of hot electron-induced ECL of the labels. r 2005 Elsevier B.V. All rights reserved.

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Cite this paper

@inproceedings{Eskola2006CompetitiveIB, title={Competitive immunoassay by hot electron-induced electrochemiluminescence detection and using a semiautomatic electrochemiluminometer}, author={Jarkko Eskola and Piia M{\"a}kinen and Laura Oksa and Kari Loikas and Mauri Nauma and Qinghong Jiang and Markus H{\aa}kansson and Johanna Suomi and Sakari Kulmala}, year={2006} }