Competitive enzyme immunoassay for human plasma fibronectin.

@article{Vuento1981CompetitiveEI,
  title={Competitive enzyme immunoassay for human plasma fibronectin.},
  author={Maarit Vuento and E-M. Salonen and Markku J Pasanen and Ulf Hakan Stenman},
  journal={Journal of immunological methods},
  year={1981},
  volume={40 1},
  pages={101-8}
}
A competitive enzyme immunoassay for the determination of fibronectin in plasma is described. An enzyme conjugate prepared by coupling alkaline phosphatase to rabbit anti-human fibronectin antibodies by glutaraldehyde was used as principal reagent. The assay was performed by coating polystyrene tubes with purified fibronectin and reacting these coated tubes with a mixture of sample and enzyme-labeled antibodies. After overnight incubation, the amount of enzyme activity associated with the tube… CONTINUE READING

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