Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.

@article{Nilsson1994CompetitiveEO,
  title={Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.},
  author={Jenny Nilsson and Per Eric Nilsson and Yvonne Williams and L. -G. Pettersson and Mathias Uhl{\'e}n and Per Ake Nygren},
  journal={European journal of biochemistry},
  year={1994},
  volume={224 1},
  pages={103-8}
}
A novel system is described for mild elution of fusion proteins by competitive elution. The approach is based on displacement of immobilized fusions containing a monovalent IgG-binding staphylococcal protein A fragment (Z) from an IgG-affinity matrix by a divalent fragment fused to a serum-albumin-binding region derived from streptococcal protein G. Using real-time interaction analysis, the binding (K(aff)) to polyclonal human IgG was found to be 3.3 (+/- 0.4) x 10(8) M-1 for divalent ZZ and 2… CONTINUE READING