Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide.

@article{Stangler2007CompetitiveDO,
  title={Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide.},
  author={Thomas Stangler and Tuyen Thi Ngoc Tran and Silke H Hoffmann and Holger Schmidt and Esther Jonas and Dieter Willbold},
  journal={Biological chemistry},
  year={2007},
  volume={388 6},
  pages={611-5}
}
We studied the interaction of the artificial 12-aa proline-rich peptide PD1 with the SH3 domain of the hematopoietic cell kinase Hck and the peptide's potency in competitively displacing HIV-1 Nef from the Hck SH3 domain. PD1 was obtained from a phage display screen and exhibits exceptional affinity for the Hck SH3 domain (K(d)=0.23 microM). Competition experiments using NMR spectroscopy demonstrate that the peptide even displaces Nef from Hck SH3 and allow for estimation of the Nef-Hck SH3… CONTINUE READING

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