Competitive binding radioassay for 5-fluorodeoxyuridine 5'-monophosphate in tissues.

@article{Murinson1979CompetitiveBR,
  title={Competitive binding radioassay for 5-fluorodeoxyuridine 5'-monophosphate in tissues.},
  author={Donald S. Murinson and T. Anderson and Herbert S. Schwartz and Charles E. Myers and Bruce Allan Chabner},
  journal={Cancer research},
  year={1979},
  volume={39 7 Pt 1},
  pages={2471-5}
}
A competitive binding radioassay has been developed for 5-fluorodeoxyuridine 5'-monophosphate, based on the tight binding of this potent inhibitor to thymidylate synthetase (EC 2.1.1.45). Unbound ligand may be separated from that bound to enzyme by precipitating the intact inhibitor-enzyme complex with trichloroacetic acid. Scatchard plot analysis using a two-site model for binding yielded apparent dissociation constants of 1.2 x 10(-11) and 1.7 x 10(-10) M from a least-squares computer fit of… CONTINUE READING