Competitive binding of small ligands to nucleobases in AP site-containing DNA duplexes.

@article{Kageyama2008CompetitiveBO,
  title={Competitive binding of small ligands to nucleobases in AP site-containing DNA duplexes.},
  author={Tomoe Kageyama and Yusuke Sato and Seiichi Nishizawa and Norio Teramae},
  journal={Nucleic acids symposium series},
  year={2008},
  volume={52},
  pages={119-20}
}
By using lumichrome (Lch) as a masking ligand, we successfully control the binding selectivity of 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) when binding to nucleobases in AP site-containing DNA duplexes (5'-TCT GCG TCC AGX GCA ACG CAC AC-3'/3'-AGA CGC AGG TCN CGT TGC GTG TG-5', X = AP site; Spacer C3, N = C or T). In solutions buffered to pH 7.0 (I = 0.11 M, at 5 degrees C), ATMND binds to cytosine and thymine with a comparable binding affinity (K(d) / nM: C: 7.7, T: 15). By contrast… CONTINUE READING