Competitive allele specific TaqMan PCR for KRAS, BRAF and EGFR mutation detection in clinical formalin fixed paraffin embedded samples.

@article{Didelot2012CompetitiveAS,
  title={Competitive allele specific TaqMan PCR for KRAS, BRAF and EGFR mutation detection in clinical formalin fixed paraffin embedded samples.},
  author={Audrey Didelot and Delphine Le Corre and Armelle Luscan and Aur{\'e}lie Cazes and Karine Pallier and Jean-François Emile and Pierre Laurent-Puig and H{\'e}l{\`e}ne Blons},
  journal={Experimental and molecular pathology},
  year={2012},
  volume={92 3},
  pages={
          275-80
        }
}
BACKGROUND The development of targeted therapies has created a need for robust molecular characterization of cancer and it has become a challenge to validate methods to ensure accuracy in tumor mutation testing. METHODS The current study was designed to evaluate KRAS, BRAF and EGFR genotyping by Competitive Allele Specific hydrolysis probes (TaqMan) PCR technology (CAST), on suboptimal formalin fixed paraffin embedded (FFPE) tumor samples. Assays were calibrated on FFPE samples and a minimal… CONTINUE READING
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Rapid detection of epidermal growth factor receptor mutations with multiplexPCR andprimer extension in lung cancer

  • Lin, C.H
  • Journal of Biomedical Science
  • 2010

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