Competitive PCR-DGGE analysis of bacterial mixtures: an internal standard and an appraisal of template enumeration accuracy.

@article{Brueggemann2000CompetitivePA,
  title={Competitive PCR-DGGE analysis of bacterial mixtures: an internal standard and an appraisal of template enumeration accuracy.},
  author={Julian Brueggemann and John R Stephen and Y. J. Chang and Sarah J. Macnaughton and George A Kowalchuk and Earl Kline and D. Carl White},
  journal={Journal of microbiological methods},
  year={2000},
  volume={40 2},
  pages={111-23}
}
Analysis of polymerase chain reaction (PCR) amplified 16S rDNA fragments from environmental samples by denaturing gradients of chemicals or heat [denaturing gradient gel electrophoresis (DGGE) and thermal gradient gel electrophoresis (TGGE)] within polyacrylamide gels is a popular tool in microbial ecology. Difficulties in acceptance of the technique and interpretation of the results remain, due to its qualitative nature. In this study we have addressed this problem by the construction and… CONTINUE READING
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