Competitive, non-competitive and cooperative interactions between substrates of P-glycoprotein as measured by its ATPase activity.

@article{Litman1997CompetitiveNA,
  title={Competitive, non-competitive and cooperative interactions between substrates of P-glycoprotein as measured by its ATPase activity.},
  author={Thomas Litman and Thomas Zeuthen and Torben Skovsgaard and Wilfred Donald Stein},
  journal={Biochimica et biophysica acta},
  year={1997},
  volume={1361 2},
  pages={169-76}
}
We have studied the interaction between verapamil and other modulators of the P-glycoprotein ATPase from membranes of CR1R12 Chinese hamster ovary cells. Four major categories of interaction were identified. (i) Non-competitive inhibition of verapamil's stimulation of enzyme activity was found with vanadate. (ii) Competitive inhibition of the ATPase was found for the pair verapamil and cyclosporin A. (iii) Allosteric inhibition with an increase in the Hill number for verapamil was found in the… CONTINUE READING